This anomaly is indicative of the larger challenge of culturing various microbial species, referred to as microbial “unculturability.” This cannot be explained by the use of agar alone or by the substitution of an alternative gelling agent, but rather by the difficulties in consistently recreating on an agar plate the multi-variable environment in which microbes grow naturally. Given such challenges, the risk of shortages, and the vulnerabilities of the agar supply chain, why is it so difficult to find suitable alternatives?
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Storage Nightmare: A CH car profile for a region can be massive (e.g., OSRM's Europe is tens of GBs, their global car profile around 200GB for just one profile). Our goal was to keep all profiles and parameters for the entire planet well under 20GB.
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